University of Leicester - 21-23 September 1998

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Provisional Programme

• Host Colloquium: Enzyme Catalysis: Structure, Dynamics and Chemistry
• Regulation in Metabolism Group/Hormone Group: Endocrine Control of Perinatal Programming in Health and Disease
• Education Group: Does Biochemistry have a Core?
• Glycobiology Group with the British Connective Tissue Society: The Biology of Hyaluronan
• Hormone Group: Oestrogen Metabolism and Breast Cancer Risk
• Industrial Biochemistry and Biotechnology Group: : Harnessing Plant Metabolites For Industry:- Tools, Systems and Implementation
• Regulation in Metabolism Group/Education Group: Modelling Metabolism
• Membrane Group: Membrane Protein Secretases
• Neuroscience Group: G protein-Coupled Receptor Signalling in the Central Nervous System
• Biochemical Immunology Group: Biosensors: Into the 21st Century
• Molecular Enzymology Group: Enzyme Catalysed Electron/Radical Transfer

The Wellcome Trust Award Medal Lecture was given by Professor John Todd

Host Colloquium:
Enzyme Catalysis: Structure, Dynamics and Chemistry

Organiser:

G. Roberts (Leicester)

Monday 21 September

Chair's Introduction:

08.45 G. Roberts (Leicester)

Speakers:

09.00 P. Sigler (Yale)

Structure and mechanism of G-proteins

09.45 K. Moffat (Chicago)

Nanosecond time-resolved crystallography

10.30 Coffee

11.00 L. Johnson (Oxford)

Protein kinase structure and mechanism

11.45 C. Bagshaw (Leicester)

Single molecule enzyme kinetics: application to myosin ATPases

12.30 Lunch

13.30 A. Leslie (Cambridge, UK)

The structure of bovine F1-ATPase: an example of rotary catalysis

14.15 B. Stoddard (Seattle)

Crystallography of intermediates in isocitrate dehydrogenase

15.00 S. Phillips (Leeds)

Galactose and amine oxidases: making the most of tyrosine residues

16.00 Poster Session

Tuesday 22 September

Speakers:

09.00 B. Shaw (Leicester)

Enzymology and Antibiotics

09.45 P. Leadley (Cambridge, UK)

Polyketide biosynthesis

10.30 Coffee

11.00 T. Maxwell (Leicester)

DNA gyrase as a drug target

11.45 J. Derrick (Manchester)

Dihydropteroate synthase: an old drug target revisited

12.30 Lunch

13.30 J. Hajdu (Uppsala)

Penicillin and cephalosporin biosynthesis

14.15 J-M. Frere (Liege)

Mechanistic diversity in -lactamases

15.00 C. Kleanthous (Norwich)

Protein antibiotics and their inhibition

16.00 Poster Session

Regulation in Metabolism Group/Hormone Group:
Endocrine Control of Perinatal Programming in Health and Disease

Organisers:

C. Whorwood (Southampton)

C. Kenyon (Edinburgh)

A. Burchell (Dundee)

Monday 21 September

Introduction:

08.50 C. Whorwood (Southampton)

Overview / Epidemiology

Speakers:

09.00 D. Barker (Southampton)

Fetal programming of adult diseases

Fetal and Placental Growth

09.45 P. Gluckman (Auckland)

The maternal, fetal and postnatal somatotrophic axes in intra-uterine growth retardation

10.30 Coffee

11.00 W. Reik (Cambridge, UK)

Embryonic growth regulation by gametic imprinting

11.45 R. Sharpe (Edinburgh)

Perinatal hormone levels and their role in normal/ abnormal development and function of the male reproductive system

12.30 Lunch

13.45 J. Hutchison (Cambridge)

Gender-specific brain formation of oestrogen in behavioural development

14.30 J. Seckl (Edinburgh)

Feto-placental growth is influenced by glucocorticoid exposure

15.15 H. Reichardt (Heidelberg)

Genetic Dissection of Glucocorticoid Receptor Function

16.00 Poster Session

Tuesday 22 September

09.00 M. Meaney (Montreal)

Early environmental events regulate neuroendocrine development

09.45 D. St Germain (New Hampshire)

Deiodinase protection of the fetus from thyroid hormones

10.30 Coffee

11.00 S. Langley-Evans (Southampton)

Intra-uterine programming of hypertension by maternal undernutrition

11.45 I. McMillen (Adelaide)

Neuroendocrine Adaptations of the Fetus to Nutrient Restriction

12.30 Lunch

13.45 S. Ozanne (Cambridge, UK)

Programming of hepatic and peripheral tissue insulin sensitivity by maternal protein restriction

14.30 M. Symonds (Nottingham)

Maternal nutrition, placental growth and programming of fetal thyroidal and adipose tissue development

15.15 J. Honour (London)

Pre-adrenarche androgens and glucocorticoids and blood pressure control

16.00 Closing Remarks

Poster Session

Education Group:
Does Biochemistry have a Core?

Organiser:

C. Smith (Manchester)

Speakers:

09.15 E. Wood (Leeds)

Overview of the biochemistry curriculum

09.50 J. Wrigglesworth (London)

Core biochemistry - The Society's view

10.30 Coffee

11.15 J. Aghion (Liege)

Core biochemistry - A European perspective

11.55 L. Jervis (Plymouth)

Core biochemistry - Lessons from biological sciences

12.35 Lunch

14.00 R. Davies (Belfast)

Core biochemistry - The necessity for chemistry

14.40 D. Boxer (Dundee)

Core biochemistry - The necessity for biology

15.15 M. Brown (SmithKline Beecham)

Core biochemistry - An industrial perspective

16.00 Poster Session

Glycobiology Group with the British Connective Tissue Society:
The Biology of Hyaluronan

Sponsors:

Glycobiology Group

British Connective Tissue Society

Seikagaku

Biomatrix

Zeneca

Pfizer

Organisers:

J. Sheehan (Manchester)

T. Hardingham (Manchester)

Monday 21 September

Speakers:

09.00 P. Weigel (Oklahoma)

Bacterial hyaluronan synthases

09.45 A. Spicer (Davis)

Mammalian hyaluronan synthase: lessons from knock-out mice

10.30 Coffee

11.00 P. Heldin (Uppsala)

Expression studies of hyaluronan synthase

11.20 C. Archer (Cardiff)

Hyaluronan signalling in embryogenesis

11.45 V. Hascall (Cleveland)

Hyaluronan metabolism

12.30 Lunch

14.00 A. Day (Oxford)

Structural regulation of hyaluronan binding to proteins

14.30 J. Sheehan (Manchester)

The Effect of water and ions on hyaluronan conformation

15.00 T. Hardingham (Manchester)

New approaches to the investigation of hyaluronan networks

15.30 E. Balazs (New Jersey)

Biomedical applications of hyaluronan

16.00 Poster Session

Tuesday 22 September

09.00 A. Pitsillides (London)

Hyaluronan in joint cavitation

09.30 M. Tammi (Kuopio)

Hyaluronan in keratinocytes

09.50 P. Noble (Yale)

Cell signalling by low molecular weight HA

10.10 R. Levick (London)

The effect of hyaluronan on trans-synovial flow

10.30 Coffee

11.00 E. Turley (Toronto)

Hyaluronan receptors: the regulation of the cytoskeleton and their role in skin wound repair

11.45 C. Knudson (Chicago)

The chondrocyte pericellular: a model for hyaluronan-mediated cell-matrix interactions.

12.30 Lunch

14.00 H. Wisniewski (New York)

TSG-6: a hyaluronan-binding protein involved in inflammation

14.45 H. Ponta (Karlsruhe)

Roles of cellular hyaluronan receptors

16.00 Poster Session

Hormone Group:
Oestrogen Metabolism and Breast Cancer Risk

Organiser:

H. Wiseman (London)

Speakers:

09.30 H. Wiseman (London)

Introduction: Importance of oestrogen, xenoestrogen & phytoestrogen metabolism in breast cancer risk?

10.00 I. Rowland (Ulster)

Oestrogen and phytoestrogen metabolism: role of gut flora

10.30 Coffee

11.15 A. Franke (Hawaii)

Phytoestrogens in human breast milk and other biomatrices

12.00 Lunch

13.45 J. Liehr (Texas)

4-Hydroxylation of oestrogens as a marker for mammary tumours

14.30 M. Dowsett (London)

Aromatase and breast cancer: control and clinical importance

15.15 M. Reed (London)

Regulation of steroid sulphatase and oestradiol 17-hydroxysteroid dehydrogenase in breast cancer

16.00 Poster Session

Industrial Biochemistry and Biotechnology Group:
Harnessing Plant Metabolites For Industry:-Tools, Systems And Implementation

Organisers:

S. Hughes (Exeter)

P. Shewry (Long Ashton)

I. Bartle (ACTIN)

A. Bunch (Kent)

Tuesday 22 September

Session1: TOOLS - to probe, change and deliver

Chair:

S. Hughes (Exeter)

09.00 S. Hughes (Exeter)

Introduction

09.05 A. van Tunen (Wageningen)

Combining genetics, genomics, IT and automated chemical analysis

09.45 M. Leech (John Innes Centre)

Genetic manipulation/plant transformation

10.25 Coffee

10.55 C. Thomas (Birmingham)

Micromanipulation Measurement of Plant Cell Mechanical Properties

11.35 P. Lapinskas (Scotia Pharmaceuticals)

Competitive production systems - cost/benefit/scale effects

Keynote lecture

12.15 N. Smirnoff (Exeter)

Ascorbate Acid biosynthesis in plants

13.00 Lunch

Session 2: SYSTEMS- from function to functionality

Chair:

P. Shewry (Long Ashton)

14.00 D. Smiley (Rothamstead)

Special Effects from Plants

14.40 A. Crozier (Glasgow)

Flavonoids as key antioxidants

15.10 R. Mithen (John Innes Centre)

Glucosinolates - more or less?

15.40 R. Casey (John Innes Centre)

Fatty acid oxidation, tasteful tunes played on the green notes

16.10 Poster Session

Wednesday 23 September

Session 3: Meeting the challenge or setting the boundaries

Chair:

C. Thomas (Birmingham)

09.00 M. Beale (Long Ashton)

Terpenoid Pathway Closing the loop,

09.40 I. Bartle (ACTIN)

Forging the net, international networking

10.00 Poster Session

11.00 T. Bach (Strasbourg)

Genetic Engineering of the plant isoprenoid pathway - all things considered

11.40 T. Slabas (Durham)

Checks and balances in the lipid pathways/networks

12.20 B. Edwards (Durham)

Sequestration and stabilisation of metabolites

13.00 Lunch

14.00 Discussion, "Have the new technologies really changed the prospects?"

C. Thomas (Birmingham)

P. Shewry (Long Ashton)

S. Hughes (Exeter)

Regulation in Metabolism Group/Education Group:
Modelling Metabolism

Organisers:

P. Quant (London)

K. Elliott (Manchester)

Tuesday 22 September

Session 1: "Experimental and theoretical approaches to modelling metabolism"

Chair:

09.00 H. Westerhoff (Amsterdam)

Speakers:

09.15 H. Westerhoff (Amsterdam)

Live control of the living cell

09.55 M. Savageau (Michigan)

Design of gene circuitry by natural selection: analysis of the lactose catabolic system in E. coli

10.35 Coffee

11.00 B. Korzeniewski (Krakow)

Theoretical studies on how ATP supply meets ATP consumption

11.40 M. Cascante (Barcelona)

New insights into metabolic pathway optimisation by analogy with industrial manufacturing processes

12.20 A. Cornish-Bowden (Marseille)

Enzyme kinetics from a metabolic perspective

13.00 Lunch

Session 2: "Experimental systems for modelling metabolism"

Chair:

14.00 A. Cornish-Bowden (Marseille)

Speakers:

14.15 J. Harwood (Cardiff)

Modelling lipid metabolism in plants - a slippery problem?

14.55 R. Iles (London)

In vivo metabolic modelling - approaches using NMR

15.35 R. Heinrich (Berlin)

Control and structural design of glycolysis: an evolutionary approach

16.10 Poster Session

Wednesday 23 September:

Session 3: "Teaching for understanding - concepts not pathways"

Chair:

09.00 K. Elliot (Manchester)

Speakers:

09.05 D. Fell (Oxford)

Traditional concepts of metabolic control mislead more than enlighten

09.45 P. Quant (London)

How to get your hands on a concept

10.15 Poster Session

11.00 J. Snoep (Amsterdam)

Introducing MCA and kinetic modelling to biologists

11.30 K. Tipton (Dublin)

Kinetics and the numerically challenged

12.00 P. Mendes (Aberystwyth)

Use of computers for modelling metabolism

12.30 Workshop & Lunch

Chair:

14.00 M. Brand (Cambridge, UK)

Speakers:

14.00 M. Watford (Rutgers)

Mrs. Spratt, young penguins and drunken elephants: teaching metabolic regulation in relation to health and disease requires a whole body approach

14.30 D. Myles (Glaxo Wellcome)

Applying the learning: the case for teaching for industry

15.00 M. Brand (Cambridge, UK)

Overview: linking research and teaching

15.30 Discussion

Membrane Group:
Membrane Protein Secretases

Sponsor:

Immunex

SmithKline Beecham

Organisers:

N. Hooper (Leeds)

A. Turner (Leeds)

Speakers:

08.45 S. Rose-John (Mainz)

The role of secretases in the shedding of cytokines and their receptors

09.30 D. Cerretti (Washington)

Characterisation of the TNF- converting enzyme (TACE) deficient mouse and its role in the shedding of cell surface proteins

10.15 Poster Session

11.00 P. Croucher (Sheffield)

Interleukin-6 receptor shedding: a role for members of the ADAM family

11.45 N. Hooper (Leeds)

Angiotensin-converting enzyme and amyloid precursor protein secretases

12.30 H. Steiner (Mannheim)

Proteolytic processing of the -amyloid precursor protein and presenilin

13.15 Lunch

14.15 J. Arribas (Barcelona)

Mechanisms controlling the shedding of transmembrane molecules

15.00 N. Bunnett (San Francisco)

Protease-activated receptors: novel functions for serine proteases

15.45 A. Turner (Leeds)

ADAMs proteases as secretases?

Neuroscience Group:
G protein-Coupled Receptor Signalling in the Central Nervous System

Organisers:

R. Challiss (Leicester)

S. Nahorski (Leicester)

K. Eidne (Edinburgh)

Chair:

S. Nahorski (Leicester)

Speakers:

09.15 S. Nahorski (Leicester)

Introduction

09.30 G. Milligan (Glasgow)

G protein-coupled receptor diversity

10.00 E. Hermans (Leicester)

Metabotropic glutamate receptor signalling

10.30 Coffee

10.55 S. Cotecchia (Lausanne)

Acute and chronic regulation of G protein-coupled receptors

11.25 A. Dolphin (London)

Ion channel modulation by G protein-coupled receptors

11.55 F. Kalkbrenner (Berlin)

Cell Cycle-Dependent Coupling of Vasopressin V1-Receptor to Gq/11 and Gi3

12.25 Lunch

Chair:

K. Eidne (Edinburgh)

Speakers:

13.45 P. Somogyi (Oxford)

Organisation of G protein-coupled receptors and ion channels at the synapse

14.15 G. Collingridge (Bristol)

Role of G protein-coupled receptors in adaptative neuronal processes

14.45 D. Hoyer (Basel)

Clinical exploitation of 5-HT receptor diversity

15.15 P. Strange (Reading)

Dopamine receptor antagonists or inverse-agonists as anti-psychotics?

15.45 K. Eidne (Edinburgh)

Summing Up

16.00 Poster Session

Biochemical Immunology Group:
Biosensors: Into the 21st Century

Sponsors:

Unilever

Biacore AB

Organiser:

S. Howell (Unilever)

Co-chairs:

S. Howell (Unilever)

C. Lowe (Cambridge, UK)

Speakers:

08.40 M. van Regenmortel (Strasbourg)

Measuring affinity interactions

09.25 H. Vogel (Lausanne)

Design of molecular biosensor surfaces for screening ligand-receptor interactions by functional assay

10.00 Poster Session

11.00 H. Hill (Oxford)

Biosensors: past, present and future

11.40 A. Turner (Cranfield)

Multichannel affinity sensors for environmental monitoring

12.20 Lunch

Co-chairs:

S. Howell (Unilever)

D. Cullen (Cranfield)

13.40 M. Malmqvist (Uppsala)

BIAcore - an affinity biosnesor for characterisation of biomolecular interactions

14.20 R. Davies (Cambridge, UK)

Cholera toxin and GM₁: a model membrane study with IAsys

15.00 Coffee

15.30 K. Mosbach (Lund)

Molecular imprinted sensors

16.10 Selected poster presentation

16.30 C. Lowe (Cambridge, UK)

Holographic Biosensors

17.10 Close

Molecular Enzymology Group:
Enzyme Catalysed Electron/Radical Transfer

Sponsors:

Heath Scientific

HiTech Instruments

Applied Photophysics

Belle Technology

Organiser:

N. Scrutton (Leicester)

Tuesday 22 September

Chair:

09.05 Opening remarks

Speakers:

09.10 F. Matthews (St. Louis)

Flavocytochromes: structures and implications for electron transfer

09.55 S. Chapman (Edinburgh)

Electron transfer in flavocytochrome b2

10.40 Coffee

11.20 G. Roberts (Leicester)

Structural studies of the flavoprotein reductase component of the P450 mono oxygenase system

12.05 A. Munro (Edinburgh)

Cytochrome P450 BM3: a paradigm for the analysis of electron transfer and its control in the P450s

12.50 Lunch

14.10 N. Scrutton (Leicester)

Electron transfer in trimethylamine dehydrogense/ electron transferring flavoprotein - a role for domain motion

14.45 V. Davidson (Mississippi)

Methylamine dehydrogenase: structure and function of electron transfer complexes

16.30 Poster Session

Wednesday 23 September

Speakers:

11.15 B-M. Sjoberg (Stockholm)

Riboneucleotide reductase - coupled electron/ proton transfer mechanisms

12.00 C. Anthony (Southampton)

PQQ-dependent methanol dehydrogense and glucose dehydrogenase

12.45 Lunch

13.45 P. Knowles (Leeds)

Galactose oxidase and TPQ-dependent amine oxidase

14.30 F. Armstrong (Oxford)

Electron transfer and coupled processes in protein film voltametry