University of Keele, 20–22 July 1999

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Meeting programme

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Meeting timetable

General information

Wednesday 21–Thursday 22 July

Membrane Group/Molecular and Cellular Pharmacology Group Colloquium
Expression and Purification of Membrane Proteins

Organizers:

C. Tate (Cambridge)

R. Grisshammer (Cambridge)

Sponsors:

SmithKline Beecham

Sanofi

GlaxoWellcome

Janssen

Pfizer

Unilever

Zeneca

Wednesday 21 July

08.45 Chair's Introduction

08.55 S. High (Manchester)

Eukaryotic membrane protein synthesis: the fundamentals

09.30 B. Miroux (Meudon-bellevue, France)

Expression and purification of the mitochondrial uncoupling proteins (UCPs): a comparative study between Escherichia coli and Saccharomyces cerevisiae

10.05 A. Ward (Leeds)

Expression of membrane transport proteins in Escherichia coli: successes and failures

10.40 Coffee

11:00 Professor Anthony Watts (Oxford)

The Morton Lecture "The need for expression expertise in solid-state NMR studies of membrane proteins: successes and wish lists"

12:00 Lunch

13.30 R. Grisshammer (Cambridge)

Functional expression in E. coli and purification of a G-protein-coupled receptor for neurotensin

14.05 S. Buchanan (London)

Overexpression and refolding of an 80 kDa iron transporter from the outer membrane of E. coli

14.40 H. Kiefer (Stuttgart)

Refolding of G-protein-coupled receptors from inclusion bodies produced in E. coli

15:15 Session Break

15.45 B. Poolman (Groningen)

Amplified expression of membrane transport proteins in Gram-positive bacteria and methods for membrane reconstitution

16.20 J. Stolz (Erlangen)

Characterization and purification of a plant sucrose transporter produced in Saccharomyces cerevisiae

16.55 J. Groves (Bristol)

Heterologous expression of the red cell anion transporter (Band 3, AE1) in yeast

17.30 Poster Session

Thursday 22 July

09:10 Chair's Introduction

R. Grisshammer (Manchester)

09.20 R. Flachmann (Heidelberg)

Expression of membrane proteins in tobacco plants

10.00 Poster Session

11.00 R. Possee (Oxford)

The use of baculovirus vectors for the production of membrane proteins in insect cells

11.35 C. Tate (Cambridge)

Improving expression of the serotonin transporter in insect cells by co-expression of molecular chaperones

12.10 W. DeGrip (Nijmegen)

Recombinant baculovirus-based functional expression of visual pigments: optimalization of expression level, purification and reconstitution

12.45 Lunch

14.00 N. Millar (London)

Heterologous expression of nicotinic acetylcholine receptors in mammalian and Drosophila cell lines: the importance of the host cell environment

14.35 P. Reeves (Cambridge, USA)

Efficient purification of rhodopsin from stable mammalian cell lines overexpressing a synthetic bovine opsin gene

15.10 Session break

15.50 M. Needham (Zeneca)

Expression of functional G-protein-coupled receptors in eukaryotic systems

16.25 H. Blasey (Serono)

Transient expression technologies, their application and scale-up: 5HT-3 receptor case study

17.00 Close